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recombinant cd22 fc chimera protein  (R&D Systems)


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    R&D Systems recombinant cd22 fc chimera protein
    Recombinant Cd22 Fc Chimera Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/siglec+2+fc+chimera+protein/Recombinant+Rat+Siglec-2%2FCD22+Fc+Chimera+Protein%2C+CF/pm41449802-200-15-19
    Average 93 stars, based on 1 article reviews
    recombinant cd22 fc chimera protein - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Cell surface ribonucleoproteins cluster with heparan sulfate to regulate growth factor signaling
    Article Snippet: MOLM-13 cells were counted and then blocked as per the manufacturer’s protocol with Human TruStain FcX (Fc block, BioLegend) for 15 minutes on ice before labeling. .. For Siglecs staining in live cells, 1 μg/mL of recombinant human IgG1 Fc (R&D Systems), Siglec-1 Fc chimera protein (R&D Systems), Siglec-2 Fc chimera protein (R&D Systems), Siglec-3 Fc chimera protein (R&D Systems), Siglec-4 Fc chimera protein (R&D Systems), Siglec-5 Fc chimera protein (R&D Systems), Siglec-6 Fc chimera protein (R&D Systems), Siglec-7 Fc chimera protein (R&D Systems), Siglec-8 Fc chimera protein (R&D Systems), Siglec-9 Fc chimera protein (R&D Systems), Siglec-10 Fc chimera protein (R&D Systems), Siglec-11 Fc chimera protein (R&D Systems), Siglec-14 Fc chimera protein (R&D Systems), and Siglec-15 Fc chimera protein (R&D Systems) were precomplexed with 0.5 μg/mL of donkey anti-human IgG AF647 (ImmunoResearch) secondary antibody in FACS buffer (0.5% BSA (Sigma) in 1x PBS) for 45 minutes on ice. .. For 9D5 and 10E4 staining, 2.5 μg/mL of 9D5 (Absolute Antibody), 1 μg/mL of anti-heparan sulfate 10E4 (amsbio), and anti-VEGF (R&D Systems) were precomplexed with 1.25 μg/mL of goat anti-Rabbit AF647 secondary antibody (ThermoFisher Scientific), 0.5 μg/mL of goat anti-Mouse AF647 secondary antibody (ThermoFisher Scientific), or donkey anti-Goat IgG AF647 (ThermoFisher Scientific) secondary antibodies in FACS buffer for 45 minutes on ice, separately.

    Recombinant:

    Article Title: Cell surface ribonucleoproteins cluster with heparan sulfate to regulate growth factor signaling
    Article Snippet: MOLM-13 cells were counted and then blocked as per the manufacturer’s protocol with Human TruStain FcX (Fc block, BioLegend) for 15 minutes on ice before labeling. .. For Siglecs staining in live cells, 1 μg/mL of recombinant human IgG1 Fc (R&D Systems), Siglec-1 Fc chimera protein (R&D Systems), Siglec-2 Fc chimera protein (R&D Systems), Siglec-3 Fc chimera protein (R&D Systems), Siglec-4 Fc chimera protein (R&D Systems), Siglec-5 Fc chimera protein (R&D Systems), Siglec-6 Fc chimera protein (R&D Systems), Siglec-7 Fc chimera protein (R&D Systems), Siglec-8 Fc chimera protein (R&D Systems), Siglec-9 Fc chimera protein (R&D Systems), Siglec-10 Fc chimera protein (R&D Systems), Siglec-11 Fc chimera protein (R&D Systems), Siglec-14 Fc chimera protein (R&D Systems), and Siglec-15 Fc chimera protein (R&D Systems) were precomplexed with 0.5 μg/mL of donkey anti-human IgG AF647 (ImmunoResearch) secondary antibody in FACS buffer (0.5% BSA (Sigma) in 1x PBS) for 45 minutes on ice. .. For 9D5 and 10E4 staining, 2.5 μg/mL of 9D5 (Absolute Antibody), 1 μg/mL of anti-heparan sulfate 10E4 (amsbio), and anti-VEGF (R&D Systems) were precomplexed with 1.25 μg/mL of goat anti-Rabbit AF647 secondary antibody (ThermoFisher Scientific), 0.5 μg/mL of goat anti-Mouse AF647 secondary antibody (ThermoFisher Scientific), or donkey anti-Goat IgG AF647 (ThermoFisher Scientific) secondary antibodies in FACS buffer for 45 minutes on ice, separately.

    FACS:

    Article Title: Cell surface ribonucleoproteins cluster with heparan sulfate to regulate growth factor signaling
    Article Snippet: MOLM-13 cells were counted and then blocked as per the manufacturer’s protocol with Human TruStain FcX (Fc block, BioLegend) for 15 minutes on ice before labeling. .. For Siglecs staining in live cells, 1 μg/mL of recombinant human IgG1 Fc (R&D Systems), Siglec-1 Fc chimera protein (R&D Systems), Siglec-2 Fc chimera protein (R&D Systems), Siglec-3 Fc chimera protein (R&D Systems), Siglec-4 Fc chimera protein (R&D Systems), Siglec-5 Fc chimera protein (R&D Systems), Siglec-6 Fc chimera protein (R&D Systems), Siglec-7 Fc chimera protein (R&D Systems), Siglec-8 Fc chimera protein (R&D Systems), Siglec-9 Fc chimera protein (R&D Systems), Siglec-10 Fc chimera protein (R&D Systems), Siglec-11 Fc chimera protein (R&D Systems), Siglec-14 Fc chimera protein (R&D Systems), and Siglec-15 Fc chimera protein (R&D Systems) were precomplexed with 0.5 μg/mL of donkey anti-human IgG AF647 (ImmunoResearch) secondary antibody in FACS buffer (0.5% BSA (Sigma) in 1x PBS) for 45 minutes on ice. .. For 9D5 and 10E4 staining, 2.5 μg/mL of 9D5 (Absolute Antibody), 1 μg/mL of anti-heparan sulfate 10E4 (amsbio), and anti-VEGF (R&D Systems) were precomplexed with 1.25 μg/mL of goat anti-Rabbit AF647 secondary antibody (ThermoFisher Scientific), 0.5 μg/mL of goat anti-Mouse AF647 secondary antibody (ThermoFisher Scientific), or donkey anti-Goat IgG AF647 (ThermoFisher Scientific) secondary antibodies in FACS buffer for 45 minutes on ice, separately.



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    Assay #2 was designed at the site between the two distinct CARs within the ORF of the vector, spanning the junction between the linker domains and the 5’-end of the FMC63 scFv of CD19. This primer-probe set was designed to be specific for the bicistronic CD19x22 CAR T cell product. A representative example of droplet readout is pictured here demonstrating that ddPCR confirms specificity of this assay to detect only the bicistronic CAR product as demonstrated with CAR positive droplets (FAM) in blue in the last column. Albumin control (VIC), depicted in green, appropriately demonstrates signal in all samples containing T cells. No CAR positive droplets (in blue) are detected in untransduced T cells or T cells transduced with either CD19 only CARs or <t>CD22</t> only CARs. NTC = No template control, negative control.
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    Image Search Results


    Assay #2 was designed at the site between the two distinct CARs within the ORF of the vector, spanning the junction between the linker domains and the 5’-end of the FMC63 scFv of CD19. This primer-probe set was designed to be specific for the bicistronic CD19x22 CAR T cell product. A representative example of droplet readout is pictured here demonstrating that ddPCR confirms specificity of this assay to detect only the bicistronic CAR product as demonstrated with CAR positive droplets (FAM) in blue in the last column. Albumin control (VIC), depicted in green, appropriately demonstrates signal in all samples containing T cells. No CAR positive droplets (in blue) are detected in untransduced T cells or T cells transduced with either CD19 only CARs or CD22 only CARs. NTC = No template control, negative control.

    Journal: Cytotherapy

    Article Title: Digital polymerase chain reaction strategies for accurate and precise detection of vector copy number in CAR T cell products

    doi: 10.1016/j.jcyt.2022.09.004

    Figure Lengend Snippet: Assay #2 was designed at the site between the two distinct CARs within the ORF of the vector, spanning the junction between the linker domains and the 5’-end of the FMC63 scFv of CD19. This primer-probe set was designed to be specific for the bicistronic CD19x22 CAR T cell product. A representative example of droplet readout is pictured here demonstrating that ddPCR confirms specificity of this assay to detect only the bicistronic CAR product as demonstrated with CAR positive droplets (FAM) in blue in the last column. Albumin control (VIC), depicted in green, appropriately demonstrates signal in all samples containing T cells. No CAR positive droplets (in blue) are detected in untransduced T cells or T cells transduced with either CD19 only CARs or CD22 only CARs. NTC = No template control, negative control.

    Article Snippet: Following staining and subsequent wash, cells were stained with Recombinant Human CD19-Fc Chimera Atto 647N Protein (R&D Biotechne, ATM9269-020, 1ug/test), Recombinant Human Singlec2/CD22-Fc Chimera Atto 488 (R&D Biotechne, ATJ1968-050, 0.5ug/test), and lyophilized T cell cocktail 6-color Kit (Biolegend, San Diego, CA) containing CD45-PE/Dazzle 594 (clone HI30), CD3 BV510 (clone UCHT1), CD4 Alexa Fluor 700 (clone SK3), CD8 Pacific Blue (clone SK1), CD45RA PE (clone KI100), and CD196 (CCR7) PE/Cy7 (clone G043H7) for 15 minutes at room temperature.

    Techniques: Plasmid Preparation, Transduction, Negative Control